Monitoring for varroa

Now that varroa is established in Australia, all hives should be regularly monitored for varroa.

If varroa is present in hives, it is important to use a standardised 300-bee sample to determine whether recommended treatment thresholds have been reached.

Rigorously monitor Varroa levels and evaluate results

Routinely monitoring Varroa levels in a portion of your colonies in each apiary is critical to making good Varroa control decisions.

When to monitor

Where Varroa is not yet established, monitoring at least 4 times per year can be done by alcohol wash, soapy water wash, or sugar shake of 300 bees per colony using methods shown in the videos.

Where Varroa is established, monitoring is recommended once a month, except during chemical treatment and winter in very cold climates.

How to monitor

Use a standard 300-bee sampling method, so that you can compare mite loads over time, and make informed decisions about treatments.

How many hives to monitor

Beekeepers with only a few hives are best to monitor all their hives regularly. Beekeepers with many hives should regularly monitor a proportion of all their hives. Where beekeepers have greater than 10 hives per apiary and are only monitoring a portion of their hives 4 times per year, it is recommended to sample colonies both from the centre and outer edges of the apiary.

  • Small apiary

    • Fewer than 10 colonies at an apiary = all colonies should be monitored for Varroa

  • Medium to large apiary, multiple apiaries

    • Greater than 10 colonies per apiary = Sample 10 or more colonies in each apiary. If possible, include colonies from the centre as well as the outer edges of the apiary.

Varroa infestations will vary between colonies, even in the same apiary, so the more colonies that can be monitored, the greater accuracy you’ll have evaluating your mite levels. In the early years of Varroa establishment, where populations have not stabilised and re-infestation is common, sampling more than 10 colonies per apiary and rotating which colonies are monitored is highly recommended.

Evaluating mite numbers

The number of Varroa you find will depend on your location, how long Varroa has been established in the surrounding environment, the time of year, your previous Varroa IPM, and other situation specific considerations. When your colony needs chemical treatment depends on the brood rearing phase your colony is in and the number of Varroa you find in the results of your monitoring (i.e. alcohol wash, soapy water wash, sugar shake).

EXAMPLE: During summer, a beekeeper alcohol washes 300 worker bees (1/2 cup or 125ml volume) from a brood frame of one hive and finds a total of 10 Varroa mites.

To calculate the Varroa %: divide the # Varroa by ÷ # bees sampled and multiply by 100.
10 Varroa ÷ 300 bees = .03 X 100 = 3% (3 mites per 100 bees).

Because it’s summer and the beekeeper’s colony is in a ‘Peak Population’ phase, a 3% Varroa infestation means Varroa control is recommended.

Quick mite infestation evaluation in the apiary

To simply evaluate Varroa infestation levels in the field, the beekeeper can count the number of mites detected per 300 worker bee sample and reference Table 2 control guidelines.

The following Varroa treatment thresholds (Table 2) were developed by the Honey Bee Health Coalition, a group of North American beekeepers, bee researchers, government agencies and other honey bee experts. Over time, Australian bee experts will develop specific Varroa treatment thresholds for Australia’s unique conditions, which may change the current recommendations.

Colony Phase Varroa %
ACTION: Wait – immediate control not needed ACTION: Control recommended
Dormant Under 1% (less than 3 mites found) Over 1% (3-5 mites found)
Population Increase Under 2% (less than 6 mites found) Over 2-3% (6-9 mites found)
Peak Population Under 2% (less than 6 mites found) Over 3% (9+ mites found)
Population Decrease Under 2% (less than 6 mites found) Over 2-3% (6-9+ mites found)

Table 2. Treatment Thresholds by Colony Phase: Varroa % = # Varroa/100 adult bees (adapted from Honey Bee Health Coalition 2022 by E. Frost). In brackets (# mites found) is the total mites found in an alcohol wash, soapy water wash, or sugar shake of 300 worker bees.

Standard 300-bee sampling methods

Varroa Mite Alcohol Wash video

Alcohol wash is considered best practice for varroa mite monitoring in Australia.

Varroa Mite Sugar Shake video

Varroa Mite Soapy Water Wash video

Alternative sampling methods

Varroa Mite Drone Uncapping video

Drone uncapping can be used to determine whether mites are present in a hive, however it does not give an indication of mite load.

If mites are found, use one of the 300-bee sample monitoring methods to determine whether the recommended treatment threshold has been reached.